Derivatives of 13-methyl-17-hydroxy-1, 2, 3, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17-tetradecahydro-15h-cyclopenta[a]phenanthren-3-ones



DERIVATIVES OF 13-METHYL-17-HYDROXY-1,2,3,

6,7,8,9,10,11,12,13,14,16,17 'ITETRADECAHYDRO-' H-CYCLOPENTA[a]PHENANTHREN-3-ONES Frank B. Colton, Chicago, 111., assignor, by mesne assignments, to G. D. Searle & (10., Skokie, 111., a corporation of Delaware No Drawing. Application August31, 1953 Serial No. 377,700 6 Claims. (Cl. 260397.47)

The present invention'relates to a new group of Organic polycyclic compounds and, more particularly, to the 13- methyl 17 hydroxy 1,2,3,6,7,8,9,10,l1,12,l3,l4,16,17- tetradecahydro 15H cyclopenta[a]phenanthren 3- ones substituted in the 17-position by a member of the .class consisting of glycolyl'and a, 3-dihydroxyethyl radicals and their lower alkanoyl esters.

. The compounds which constitute this invention can be represented by the structural formula O 0,O(lowera1kyl) radicals and R is a member of the class consistingof hydrogen and OC (lower alkyl) radicals.

The claimed compositions are valuable in providing medicinal agents. They produce some of the therapeutic given in degrees centigrade C.) and relative amounts of materials in parts by weight. I Example 1 7 A stirred solution of 10.6 parts of 3-methoxy-l3- methyl 1,4,6,7,8',9,11,12,l3,l4,l6,17 dodecahydro- 15H cyclopenta[a]phenanthren 17 one in 700 parts of anhydrous ether and 45 parts of dry toluene is cooled to.0 C. and saturated with dry acetylene. While a slow stream of acetylene is passed through the reaction mixture, a solution of 20 parts of potassium t-amylate in 135 parts of anhydrous pentanol is added in the course of 15 minutes with stirring. Passage of acetylene and stirring are continued for an additional 4 /2 hours. ing at 0 C. for 16 hours, the mixture is washed with aqueous ammonium chloride solution until the aqueous phase is neutral, then with water and saturated sodium chloride solution. The organic layer is dried over anhydrous sodium sulfate, filtered and concentrated under a vacuum to a residue of about 250 parts. 500 parts of petroleum ether are added and, after standing at 0 C. for

e an hour, the mixture is filtered. The collected precipitate determined in a 1% chloroform solution is [0c] +65.

3200 parts of methanol and .1000 parts of water areadded 240 parts of concentrated hydrochloric acid. Refluxing is eifects of the mineralocorticoid adrenal hormones, such :3:

as sodium retention and life maintenance, but lack the undesirable side effects which limit the clinical utility of the naturally occurring steroids. The specificity of the activity of the claimed compositions is demonstrated by the fact that while the l3-methyl-17-(fl-acetoxyacetyl)- 1953, issued as U. S. Patent 2,704,768, of whichthe present application is a continuation-in-part. I Additional useful procedures appearfrom the examples below.

; These examples illustrate the compounds of my invention and the methods for their preparation, However, the

invention is not to be construed as limited by the details set forth in spiritor in scope. It willbe apparentto those skilled in the art that many modifications in materials and methods may be practiced without departing fron'ithe invention. In each of these examples, temperatures are :Patent 2,655,518, and Serial No. 357,377, filed May 25,

is recrystallized from ether. The resulting 3-methoxy-l3- methyl 17 ethynyl -,1,4,6,7,8,9,11,12,13,14,16,17 dodeca hydro 15H cyclopenta[a]phen'anthren 17 o1 melts at'about 181-182 C. The molecular rotation as An additional amount of this product can be obtained from the mother liquors by concentration under vacuum followed by addition of petroleum ether.

Example 2 methyl 17 ethynyl '1, 4,6,7,8,9,11,l2,13,l4,l6,17 dodecahydro 15H .cyclopenta[a]phenanthren 17 01 in '204 C. The molecular rotation, as determined in a 1% chloroform solution, is [u] =22.5. The ultraviolet absorption spectrum of a methanolic solution shows a maximum at 24.1 millimicrons with a molecular extinction coeflicient of 17,100. I

i I Exampled" tion of one molecule of hydrogen the reduction is stopped and the mixture is filtered. The filtrate is concentrated under vacuum to about 500 parts, diluted with 3000 parts of ether and washed with normal hydrochloric acid until a Congo red test shows an acidic reaction. The solution is washed sucessively with water, 5% sodium bicarbonate, water and saturated sodium chloride solution. The ether extract is dried over sodium sulfate, concentrated on the steam bath to about 500 parts and diluted with 800 parts of petroleum ether. .After storage at 0 C..for 16 hours, the product is collected on a filter, dried and crystallized from'a mixture of ethyl acetate and petroleum ether. The 13-methyl-17-vinyl-17- Patented June 24, 1958 After stand To'a refluxing solution of 47.5 parts of 3-methoxy-13- 30,200. I compound. has the hydro ry l,2,3,6,7,8,9,10 ,',11,l2,13,14 ,16,17 -tetradecal 1ydro-ISH-cyclopentaEa]phenanthren-3-one thus obtained melts at about 169-171 C. The molecular rotation of an a lcoholic solution is [a] ==-+36. r r Example 4 A-solution of 47.3 parts 'ofphosphorus .tribromide in 645 parts of anhydrous ethanol-freechloroform is added dropwise to a solution of 142.9 parts of '13-methyl-17- vinyl-17-hydroxy 1,2,-3,6,7,8,9,10,11 ,12 l3,14, 16,17 tetradeezihydro-ISH-cylopehtafa]phenanthrer|-3-one in 2250 parts of chloroform and parts of pyridine, maintained' at'-20f C; After standing at room temperature fol-316 hours, the'mixtureis treated with chloroform and thentsuccessively with .dilute hydrochloric acid,dilute sodiur'ri bicarbonate solution and finally with water. After dryingfover anhydroussodium sulfate, the chloroform is stripped ofi, leavingua's a residue the 17 -(fl-bromoethylide'ne)-13 methyl l,2,3,6,1,8;9,10;1 1,12,13,14,1 6,11 tetradecaliydro-H-cyclopenta[aJphenzinthren-B-one. L

45 parts of l7-(flebromoethylidene)-13-n1ethyl-l,2,3,6, .7,8,9,10,1 1,12,13,14,16,17-tetradecahydro-15H cyclopenta[a lphenanthren-3-one are treated with 400 parts of freshly fused potassium acetate and refluxed for 5 hours in 3200 parts of dry acetone. After cooling the precipitate is removed by filtration and the acetone is distilled in vacuum under nitrogen. The residue is extracteldby refluxing with boilingpetroleumuether and, after. stripping 4 Example 5 To a solution of 25 parts of 17-(fl-acetoxyethylidene)- 13-methyl-1,2,3,6,7,8,9,10,11,12,13,14,16,17 tetradecahydro-ISH-cyclopenta[a1phenanth1en-3-one in 200 parts of tertiary butanol areadded 0.27 part 'ofosmium tetroxide in 16 parts of tertiary butanol, followed immediately by olthc .solventinvacuo, the residue is chromatographed over #1500 parts of silica gel. Elution with: 313% solution' ofiethyl acetate in benzene, evaporation of the solvent frQm the-eluateand, crystallization ofthe residue from.

aqueous acetone and-petroleum ether yields IS-methyldflvinyl-,1,2,3,6,7,8,9,10,11,l2,13.14;dodecahydro-15H-cyclopenta[a]phenanthren-3=one, melting at about 100-101 CL. The molecular rotation ofan 0.66% chloroform solu-.

tionis' [a] 4 1110.5 The ultraviolet absorption: spectrum of a methanolic solutionshows a maximum at 237 millimicrons with a molecular extinction. coeflicient of structural formulav Elution of the chromatography column witha 10% solution of ethyl acetate in benZeneQeVaporatiOn of the.

241 millimicrons with a molecularfextinction' coefficient 7 of 917,800. This compound has the: structural formula orr 7 r t mse m on.

60 parts of a 3.27-N hydrogen peroxide solution in tertiary butanol. In the-course of the following two hours, a solution of 1.25 parts of osmium tetroxide in parts of tertiary butanol is added. After standing at room temperature for 24 hours, the mixture is treated with 1500 parts of water and concentrated in vacuum at room temperature until about 320 parts of distillate have been collected. The residue is extracted with ethyl acetate and the extract is washed with water, dried over sodium sulfate, filtered, and evaporated to dryness. The residue is taken up in 1000gparts of methanol andrefluxed for 30 minutes with a solution of 9 parts of sodium sulfite in 200 parts of water. The reaction mixture is concentrated to about one-half of its original volume under nitrogen and extracted withethyl acetate; This extract is washed with water, dried over sodium sulfate and evaporated. The residue contains'a mixture of 13-methyl-17-glycolyl- 17-hydroxy-1,2,3,6,7,8,9,10,11,12,13,14,16,17-tetradecahydro-ISH-cyclopenta[alphenanthren-3-one and 17-(a,,sdihydroxyethyl)-l7-hydroxy-13-methyl 1,2,3,6,7,8,9,10,- 11,l2,13,14,16,17-tetradecahydro 15H cyclopentaEphenanthren-S-one. These compounds have the structural formulae CHsOH CHaOH In addition the mixture contains a third compound which is apparently 4,5-dil1ydroxy-13-methyl-17-(fl-hydroxyethylidene)perhydro 15H cyclopentaialphenanthren-3-one of the structural formula CH: V

CHgO-QQCH:

The" above residue is dissolvedin 35 parts of pyridine and '35 parts of acetic anhydride and kept at room temper ature for 15 hours. Ice and, 2 hours laterpwater is added and the mixtureis extracted with ethyl acetate. This. extract 1 is washed with. dilute, hydrochloric acid, L sodium bicarbonate and water. After drying oversodium sulfate, the. extract is "evaporatedunder vacuum and the residue is chromatographed over '250 parts of silica gel. Thecolumniselutedfirst-with 1500 aras: a 10% solution of 'ethyl acetate in benzene. Elution with 500 parts each of a 10% and a 15 solution of ethyl* acetate in benzene yieldsunreacted starting material. The column is next washed with an additional 500 partsof a 1s% solutionof ethyl acetate in benzene. Elution with "a? further 500-part'portion of such a 15% 'solution and evapether and then from ether, forms crys'tals melting at about 185187 C. This material gives apositive blue tetrazolium test and does not have a specific absorption maximum in the ultraviolet spectrum between 220 and 330 millimicrons. The infrared spectrum shows maxima at about 2.78, 5.78, 6,9, 7.3, 8.06, 8.79, 9.21, 9.5, 9.75, 10.3, 10.55, 10.8 and 11.3 microns. The compoundis apparently 4-acetoxy-5-hydroxy-13-methyl-17-(B-acetoxyethylidene)perhydro-ISH-cyclopenta[alphenanthren-B-one of the structural formula CHlCl-O C OH! CHsCQ Elution of the column with 2000 parts of a 20% ethyl acetate in benzene solution, concentration of the eluate under vacuum and repeated recrystallizations from aqueous methanol and then from a mixture of ethyl acetate and petroleum ether yields the 13-methyl-17-(p-acetoxyacetyl) 17 hydroxy 1,2,3,6,7,8,9,10,11,12,13,14,16,17 tetradecahydro 15H cyclopenta[a]phenanthren 3 one melting at about 233-235 C. A 1% chloroform solution shows a molecular rotation of [a] +88. The ultraviolet absorption spectrum shows a maximum at 242 millimicrons with a molecular extinction coeflicient of 17,700. ?This compound has thestructural formula N CH: I. I

CHsO-rOC-Cflt Further elution of the chromatography column with 2000 parts of a 20% solution and 1000 parts of a 30% solution of ethyl acetate in benzene and concentration of the eluate yields the 17-(a,p-diacetoxyethyl)-17-hydroxy- 13 methyl 1,2,3,6,7,8,9,10,11,12,13,l4,16,17 tetradecahydro-ISH-cyclopenta[aJphenanthren-3-one which, recrystallized from ethyl acetate and petroleum ether, melts at about 194196 C. The molecular rotation of To a solution of 26 parts of 13-methyl-17-(p-acetoxyacetyl) 17 -hydr oxy- 1,2,3,6,7,8,9,10,11,12,13,14,16,17 tetradecahydro 15H cyclopenta[a]phenanthren 3 one in 4000 parts of methanol are added 700 parts of a 0.1-N sodium hydroxide solution by gradual addi- 6 tion in the course of 3 minutes. The reaction mixture is maintained at 25 C. for 20 minutes and then treated with 15,000 parts of water. After standing at 25C. for an additional 30 minutes, the mixture is neutralized with 20% aqueous acetic acid, 7000 parts of solventare distilled ofi under vacuum and the residue is stored at 0 C. for 15 hours. The precipitate is collected on a filter, washed with water and recrystallized from a mixture of ethyl acetate and petroleum ether. An additional yield is obtained by extracting the mother liquor with ethyl acetate. The extract is washed with water and saturated sodium chloride solution, dried, evaporated and recrystallized from ethyl acetate and petroleum ether. The 13- methyl 17 glycolyl 17 hydroxy 1,2,3,6,7,8,9,10,11,

12,13,14,16,17 tetradecahydro 15H cyclopenta[a] phenanthren-3-one thus obtained melts at about 177180 C. The molecular rotation of an 0.816% chloroform solution is [a];,=+59.2.

Example 7 A solution of 10 parts of 17-(fl-acetoxyethylidene)-l3- methyl 1,2,3,6,7,8,9,10,11,12,13,14,16,17 tetradeca 'hydro15H-cyclopenta[alphenanthren-3-one in 700 parts of ether, dried by distillation over sulfuric acid, is cooled to 0 C. and treated with a solution of 8 parts of osmium tetroxide in 700 parts of dry ether. The reaction mixture is permitted to standat room temperature for 5 days after which the ether is removed by decantation and the black residue is taken up in 1000 parts of methanol and treated with a solution of 3 parts of sodium sulfite in 50 parts of water, After refluxing for one hour, the reaction mixture is diluted'with water and extracted with ethyl acetate. This extract is washed with water and with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulfate and evaporated under vacuum. The resi .due is dissolved in 500 parts of a l-N methanolic potassium hydroxide solution and refluxed for 15 minutes. The mixture is then diluted with water and extracted with ethyl acetate. This extract is washed with saturated sodium chloride solution, dried and evaporated to yield 13- methyl 17 (c p dihydroxyethyl) l7-hydroxy-l,2- 3,6,7,8, 9,10,'11,l2,13,14,16,17 tetradecahydro 15H-cyclopenta[alphenanthren-3-one. This compound is identical with the one obtained by chromatography of the nonacetylated reaction mixture obtained in Example 5. The compound shows infrared absorption maxima at 2.9, 5.84, 6.0, 6.19, 7.88, 8.22, 9.49, 10.0, 10.30, 11.10, 11.30, and 11.70 microns.

\ Example 8 HOH OH 7 Example 9 To, a solution of 20 parts of l3-methyl-l7-(qrhydroxy- 13 acetoxyethyl) 17 4 hydrox y 1,2,3,6,7,8,9,10,11,12,- 13,14,l6;17#tetradecahydro 15H cyclopentalalphenanthren-3-one in 150 parts of pyridine is added a suspension of 15.5 parts of chromium trioxide in 150 parts of pyridine. The reaction vessel is closed and the contents are mixed intimately and allowed to stand at 25 C for12 hours; Themixture is then poured into water .and e ttracted with ethyl acetate. This-extract is Washed successively with dilute hydrochloric acid, sodium bicarbonate and water, dried over F anhydrous sodium sul fate and evaporated. The residue is recrystallized from' a mixture of ethyl acetate and petroleum ether. The 13.- methyl '17 (B acetoytyacetyl)-17-hydroxy 1,2,3,6,- 7,8,9 ,10,1l,12,l3,l4,l6,17 tetradec ahydro l5H-cyclopenta[a]phenanthren-.3-one thus obtained melts at about 235 C.

I claim:

1. A compound of the structural formula GHQOR CH: CH:

OH: on, $11 11- H,

HIV Cfi 0Q, wherein X is a member of the class consisting .of =;O,

H QE and 1o =0 on, on. on y Cz \CH: on, AH n-im:

15 c l o c CH C 1 5. 13 methyl 17 (p acetoxyacetyl) 17 hy- 20 droxy 1,2,3,6,7,8,9,10,11,12,13,14,16,17 tetradecahydro-15H-cyclopenta[a] phenanthren-S-one.

6. A compound of the general formula CHiOR 1101i 25 OH wherein'R is a member of the class'consistin gofhydrogen and acetyl.

Belgium Nov. 26. 1951 

1. A COMPOUND OF THE STRUCTURAL FORMULA 